质粒名称: | 5aa SunTag VP64 EVD |
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出品公司: | 爱迪基因 |
目录编号: | 115483 |
存储实验室: | Steven Jacobsen |
载体骨架基本信息 |
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载体名称: | pMOA34 |
载体出品公司: | -- |
载体抗性: | 大观霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 植物表达,CRISPR,合成生物学 |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS,5aa SunTag VP64 EVD |
物种来源: | -- |
插入大小: | 15407 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | 5aa SunTag VP64 EVD was a gift from Steven Jacobsen (Addgene plasmid # 115483 ; http://n2t.net/addgene:115483 ; RRID:Addgene_115483) |
参考文献部分: | Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 5aa SunTag VP64 EVD 26054 bp ds-DNA circular SYN 21-MAR-2019 DEFINITION CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs. ACCESSION . VERSION . KEYWORDS 5aa SunTag VP64 EVD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 26054) AUTHORS Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE TITLE Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. JOURNAL Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. PUBMED 30760722 REFERENCE 2 (bases 1 to 26054) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 21, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..26054 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 4657..4712 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" CDS 4726..8829 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 8833..8859 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 8878..8898 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 8905..8925 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 8989..9045 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9061..9117 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9133..9189 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9205..9261 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9277..9333 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9349..9405 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9421..9477 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9493..9549 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9565..9621 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" CDS 9637..9693 /codon_start=1 /product="GCN4 peptide optimized to improve solubility while preserving binding to an scFv-GFP fusion protein (Tanenbaum et al., 2014)" /label=GCN4_v4 /translation="EELLSKNYHLENEVARLKK" terminator 9741..10448 /label=OCS terminator /note="octopine synthase terminator" promoter complement(10481..10499) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(10482..10499) /label=SP6 /note="SP6 promoter, forward primer" misc_feature 10542..10566 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" oriT 11128..11237 /note="incP origin of transfer" mobile_element 11297..12064 /mobile_element_type="insertion sequence:IS1" /note="prokaryotic transposable element" CDS 12689..13837 /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" primer_bind complement(15026..15043) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(15197..15785) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(15277..15296) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS 16882..17670 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 18345..18876 /label=oriV /note="incP origin of replication" rep_origin 18353..18967 /label=oriV /note="origin of replication for the bacterial F plasmid" misc_feature 19067..19091 /label=LB T-DNA repeat /note="left border repeat from octopine Ach5 T-DNA" terminator 19165..19417 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(19445..20470) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(20524..20703) /label=NOS promoter /note="nopaline synthase promoter" misc_RNA complement(21161..21236) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_RNA complement(21718..21793) /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS complement(22551..22601) /codon_start=1 /product="nuclear localization signal from the human T-cell leukemia virus type 1 (HTLV-1) Rex protein" /label=Rex NLS /translation="PKTRRRPRRSQRKRPPT" CDS complement(22629..22790) /codon_start=1 /product="B1 domain of Streptococcal protein G " /label=GB1 /note="effective as a solubilizing fusion partner (Cheng and Patel, 2004)" /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD ATKTFTVTE" CDS complement(22821..22841) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(22866..23015) /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS complement(23055..23765) /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" primer_bind complement(23095..23115) /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" primer_bind 23710..23738 /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" CDS complement(23856..23882) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac 61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt 121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc 181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg 241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt 301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt 361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg 421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta 481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg 541 ttaaaagctc tgttgagcta gactcatatg catcctagga aacaagttgt aatgagttgc 601 tggcctctct tgggattgtt catcaaagta gctgtcccta cacaccacaa cagaatggtg 661 ttgtggagag aaagcacaga cacatccttg agatggcaag ggcacttaag tttcagagtg 721 gtgtacctac caggttttgg ggagactgtg tcaagactgt tgtatacctg atcaatagac 781 tccctactcc aatcttacaa ggcaaatgtc catatgaact actctatcag aaacatgcca 841 agcttgatca cttgagagtg tttgggtgtc ttgcttttgc agcaacactg cccaaaggtg 901 acaaacttgc acctagagcc aagcgaacca tcttcattgg ttattctgag actcaaaagg 961 gttacaggtt gtatgacttg gataataagg tgatcatagt gagcagggat gtagttttca 1021 gagagtttca gttccctttc aaagagtcca tctcccatga acctgatatg ttcactcgag 1081 caggttcact tagctctgaa gatacagctt tgcagctagt gtataatgat atttttcctt 1141 ccagctttca tactgatgat gatgatactc cagttcttat tcctgacatt gtagaggcca 1201 atgaggatat cactttgggg actcattcta cccaaaatca tatcacttca gctgatgctg 1261 caccttcagg ccacctgcat gctcctgcag agtcagttga gtcagctgag cctgacctag 1321 caaatgctga atttgagcac catactgctg tagctgatcc ctctctagtt ccagcaaacc 1381 tgcacactag acctaaacgc aatgcaggtc ctcccatctg gctcaaggac tttgtgacac 1441 tgaacaaagg ctctagggat gttccatacc ctatagccaa ctatgtttcc tatgatcact 1501 tgcctgttca ctatcaagct tatttgagtg ctttctccac ttatactgaa cctaaatcct 1561 tcaaagaagc agctcaagat gagaaatgga tggaggccat gtcccttgag atacaggctc 1621 ttgaggataa taacacctgg gagattgtcc ctttaccccc tggtaaacag cctatagggt 1681 ccaaatgggt gtacaaaatt aaatacaaag ctaatggtga agttgacagg tttaaggcaa 1741 ggctagtggc caagggatac actcagcaag aaggccttga ctaccatgaa actttttctc 1801 cagtggccaa aatggtcact gtaaaagctg tcatatctgt tgctgcttcc aagggctggt 1861 tccttttcca gatggatgtc aacaatgctt ttctacaagg tgacctcatg gaagaagttt 1921 atatgtctct gcctcagggt tttcacagcc aaggggagac caaagtgtgc aggctgttga 1981 atccctctat ggtttaaagc aagcatcaag gcagtggaac atcaagctta ccactgtcct 2041 tatgcaggct ggttttatgc aaagtgctta tgatcactcc ttgtttacca aaagagaggg 2101 gactgacctt gtcataatcc tgatttatgt ggatgatctg ttaataacag gcagcagcaa 2161 tgttttgatt tcagaagcaa aggcaaccct gcatcagcat ttcaagatga aagatttggg 2221 agaactaaaa tacttcttag gcattgaggt gctgagatca gaaaagggaa tcctactgaa 2281 ccagaggaag tatgcactgg aactgatatc aggtgttggt ctgggaggct gcagaccagt 2341 gtcaacccca atggaacaga accaaaggct gactactgta gaatatgaca agcacttagg 2401 aaagactgat gatgcagagt tagaggatgt gggttcctat cagagactgg tgggaaagct 2461 tctctacttg acaatcacaa ggccagatat cagctttgca gtgcaggtgc tgagtcaatt 2521 catgcagcaa cccaaacagt cacacctgga agcagcattg agggttgtga agtacataaa 2581 aggttctcca ggtgttagga agcggccgca tgcatatgag tctagctcaa cagagctttt 2641 aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag aaagagattc 2701 cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa gcataaaatc 2761 ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac ttgacaagca 2821 taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa tgatcttcct 2881 ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt ctacttccag 2941 cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc ctgtatgatc 3001 gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc catcaaagtt 3061 gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc gacggtgttg 3121 tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca tctgcatctt 3181 cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc accagctcgt 3241 ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca ttcttcagat 3301 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag 3361 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc 3421 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa 3481 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc 3541 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc 3601 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag 3661 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact 3721 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat 3781 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc 3841 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa 3901 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa 3961 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg 4021 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta 4081 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta 4141 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtattttct tcttcttctt 4201 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa 4261 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta 4321 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt 4381 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc 4441 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg 4501 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg 4561 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagggatca tcaaaaagtt 4621 tgtacaaaaa agcaggctct ttaaagtatt tttacaacaa ttaccaacaa caacaaacaa 4681 caaacaacat tacaattact atttacaatt acaaaaaaag ttaacatgga caagaagtac 4741 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac 4801 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag 4861 aacctgatcg gcgccctgct gttcgacagc ggagaaacag ccgaggccac ccggctgaag 4921 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc 4981 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc 5041 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag 5101 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc 5161 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg 5221 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc 5281 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc 5341 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg 5401 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc 5461 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag 5521 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag 5581 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc 5641 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac 5701 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag 5761 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta catcgatggc 5821 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc 5881 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc 5941 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg 6001 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc 6061 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg 6121 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc 6181 gccagcgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag 6241 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc 6301 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa 6361 aaagccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa 6421 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat 6481 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac 6541 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg 6601 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac 6661 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag 6721 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc 6781 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa 6841 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc 6901 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac 6961 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga 7021 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa 7081 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag 7141 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac 7201 caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc 7261 tttctgaagg acgactccat cgataacaaa gtgctgactc ggagcgacaa gaaccggggc 7321 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag 7381 ctgctgaatg ccaagctgat tacccagagg aagttcgaca atctgaccaa ggccgagaga 7441 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg 7501 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag 7561 aacgacaaac tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat 7621 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac 7681 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa 7741 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc 7801 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt 7861 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca 7921 aacggcgaaa caggcgagat cgtgtgggat aagggccggg actttgccac cgtgcggaaa 7981 gtgctgtcta tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc 8041 agcaaagagt ctatcctgcc caagaggaac agcgacaagc tgatcgccag aaagaaggac 8101 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg 8161 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg 8221 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag 8281 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag 8341 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa 8401 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg 8461 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caaacactac 8521 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct 8581 aatctggaca aggtgctgag cgcctacaac aagcacagag acaagcctat cagagagcag 8641 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag 8701 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc 8761 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg 8821 ggaggcgacg cctatcccta tgacgtgccc gattatgcca gcctgggcag cggctccccc 8881 aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt 8941 gggagcaacg gcagcagcgg atccaacggt ccgactgacg ccgcggaaga agaacttttg 9001 agcaagaatt atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga 9061 gaagaactgc tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg 9121 agcggaagtg gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga 9181 ctaaagaaag ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac 9241 gaagtggctc ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac 9301 cacctcgaaa atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta 9361 tccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga 9421 gaggaactgc tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga 9481 tcgggcagtg gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga 9541 ttaaagaaag gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat 9601 gaggtagcga gactaaaaaa ggggagtggt tctggcgagg aattgctatc gaaaaattat 9661 catcttgaga acgaagttgc taggctcaaa aagggctcag gctcaggcta acccgggggc 9721 gcgccatgat aactagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga 9781 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc 9841 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat 9901 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa 9961 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca 10021 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg 10081 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct 10141 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc 10201 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa 10261 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc 10321 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct 10381 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg 10441 ctcagcagga ccggcatgca agctagctta ctagtgatat tctatagtgt cacctaaatc 10501 tgcggccgcg gtaccactag tacgcgtagg cctgagctct ttgacaggat atattggcgg 10561 gtaaacgata tcggcacctt cgaccgcctg ttccgcagag gtgcaggcct cgatctgaaa 10621 cccgaaccgc tggagattgc gggagcagcg agcagtagcc tcggggtcga tgtcgtaaag 10681 tcgtatccga tcgacgccga tcagcgcctt gaaggccaaa gcctggaact cactttgggc 10741 accgttgccg atcagcgcca tcgtgcgcga atctttacgg gccagatact ttgccgcgat 10801 cgcggaggtc gcggccgttc gcaaggccgt caggattgtc atttccgaca gcagcagcgg 10861 atagccgcta tcgacatcgg agagcacgcc gaacgcggtt accggcctct tcatcgggaa 10921 tgcgcgcgac cttcagcatc gccggcatgt ccccctggcg gacgggaagt atccagctcg 10981 acctcgacgg atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc 11041 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt 11101 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag 11161 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc 11221 tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga 11281 aaccaagcca accaggggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt 11341 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg 11401 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta 11461 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca 11521 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc 11581 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca 11641 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc 11701 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc 11761 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc 11821 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg 11881 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct 11941 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct 12001 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat 12061 tacccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga 12121 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc 12181 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc 12241 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg 12301 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag 12361 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt 12421 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc 12481 catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa 12541 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta 12601 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg 12661 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag 12721 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac 12781 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac 12841 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc 12901 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac 12961 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc 13021 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa 13081 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc 13141 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc 13201 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt 13261 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg 13321 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg 13381 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac 13441 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg 13501 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga 13561 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa 13621 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc 13681 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg 13741 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc 13801 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc 13861 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg 13921 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac 13981 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac 14041 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg 14101 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat 14161 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag 14221 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa 14281 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc 14341 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc 14401 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc 14461 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct 14521 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc 14581 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc 14641 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc 14701 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca 14761 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg 14821 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg 14881 cgactcgaac ctacagttgt ttccttactg ggctttctca gccgggatgg cgctaagaag 14941 ctattgccgc cgatcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 15001 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 15061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 15121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 15181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 15241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 15301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 15361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 15421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 15481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 15541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 15601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 15661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 15721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 15781 atcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 15841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 15901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 15961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 16021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 16081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 16141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 16201 ttaaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtgcc aaaagccgcg 16261 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct 16321 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat 16381 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag 16441 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac 16501 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa 16561 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt 16621 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac 16681 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg 16741 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc 16801 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc 16861 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta 16921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 16981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 17041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 17101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 17161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 17221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 17281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 17341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 17401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 17461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 17521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga 17581 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa 17641 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc 17701 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg 17761 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca 17821 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt 17881 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac 17941 aactctgaga gcaaatacga taatagttca tccaattacg acaatagtcg caacggaaat 18001 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc 18061 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa 18121 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata 18181 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac 18241 tagcctgctc tctaataaaa tgttaggagc ttggctgcca tttttggggt gaggccgttc 18301 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt 18361 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg 18421 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg 18481 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc 18541 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg 18601 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc 18661 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg 18721 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc 18781 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc 18841 caagttttcc gcgaggtatc cacaacgccg gcggccggcc gcggtgtctc gcacacggct 18901 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg 18961 caaccagccc ggtgagcgtc ggaaagggtc gaggaattcc gcctaaccgg ctcagttctg 19021 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc aggatatatt 19081 caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag tatgatggtc 19141 ccatcgataa gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt 19201 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga 19261 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat 19321 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca 19381 atcttaagaa actttattgc caaatgtttg aacgatcggg gatcgatccc ggtcggcatc 19441 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta 19501 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga 19561 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat 19621 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg 19681 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct 19741 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat 19801 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga 19861 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc 19921 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag 19981 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt 20041 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg 20101 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag 20161 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc 20221 tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat 20281 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac 20341 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt 20401 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag 20461 gctttttcat atcttattgc ccccctagag tcgagatctg gattgagagt gaatatgaga 20521 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat 20581 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt 20641 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg 20701 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg 20761 actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa 20821 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg 20881 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc 20941 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta 21001 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt 21061 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagcttgca tgcctgcagg 21121 tcgactctag acacggggtg gtttggttta aacaaaaaaa gcaccgactc ggtgccactt 21181 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaaccata 21241 ttctttcatg gtatcacaat cactacttcg actctagctg tatataaact cagcttcgtt 21301 ttcttatcta agcgatgtgg gacttttgaa gattgttttc aacttaaatg ggcctatata 21361 agaaatacta ttgttctttc ccatataaat gggcctgctt ctcttctttc agattcccag 21421 gggccttttg aagattatct tcatatctta agaatgaaga tgttttattc aatcaaattc 21481 ttgaaggttc gatgcctaat cattctaatc ctgggacaaa ctatgaaaca agatacaaaa 21541 actccgaatg gaaagttaaa aagaagaaaa cgaaagctac ggttcaagaa aatgtaagct 21601 gataaacaaa aaaaaactgt atgaacgaag aagaagaaaa aaagctaaga agaaatgatg 21661 tattgtgcgg aaggcaagtc gagtttccgt tgttcaacga aagcttgttt aaaaaaagca 21721 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt 21781 ctagctctaa aacacagata atagctttat tcacaatcac tacttcgact ctagctgtat 21841 ataaactcag cttcgttttc ttatctaagc gatgtgggac ttttgaagat tgttttcaac 21901 ttaaatgggc ctatataaga aatactattg ttctttccca tataaatggg cctgcttctc 21961 ttctttcaga ttcccagggg ccttttgaag attatcttca tatcttaaga atgaagatgt 22021 tttattcaat caaattcttg aaggttcgat gcctaatcat tctaatcctg ggacaaacta 22081 tgaaacaaga tacaaaaact ccgaatggaa agttaaaaag aagaaaacga aagctacggt 22141 tcaagaaaat gtaagctgat aaacaaaaaa aaactgtatg aacgaagaag aagaaaaaaa 22201 gctaagaaga aatgatgtat tgtgcggaag gcaagtcgag tttccgttgt tcaacgaaag 22261 ctttcctaaa cgtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta 22321 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca 22381 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag 22441 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg 22501 ccaaatgttt gaacgatcgg ggaaattcga gctcaaactt acggccatgg cgtaggcggc 22561 cttttccgtt gtgatcttcg cggtctcctc cttgtttttg gactagtacc accaccgcta 22621 ccaccacctt cggttaccgt gaaggttttg gtagcgtcgt cgtaggtcca ttcaccgtca 22681 acaccgttgt cgttagcgta ctgtttgaaa actttttccg cggtagcagc gtcaacagct 22741 tcggtggtgg tttcaccttt cagggtttta ccgttcagga taagcttgta ctcttcggtc 22801 cgagatcctc ctcccgtacg gacctttctc ttcttctttg gagcccctcc gccaccgcca 22861 ctacctagca tatctagatc aaagtcatct agggcatcgg agcccaacat atctaaatcg 22921 aagtcgtcca atgcatctga gcctaacatg tctaggtcga agtcgtctaa cgcgtctgaa 22981 ccaagcatat ctaagtcaaa atcgtctaaa gcatcccctc cgccaccgcc cgtacgaccg 23041 gtccgacctc cacctttgta gagctcatcc atgccatgtg taatcccagc agcagttaca 23101 aactcaagaa ggaccatgtg gtcacgcttt tcgttgggat ctttcgaaag gacagattgt 23161 gtcgacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt 23221 tgataatggt ctgctagttg aacggaacca tcttcaacgt tgtggcgaat tttgaagtta 23281 gctttgattc cattcttttg tttgtctgcc gtgatgtata cattgtgtga gttaaagttg 23341 tactcgagtt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ctttaactcg 23401 atacgattaa caagggtatc accttcaaac ttgacttcag cacgcgtctt gtaggtcccg 23461 tcatctttga aagatatagt gcgttcctgt acataacctt cgggcatggc actcttgaaa 23521 aagtcatgcc gtttcatgtg atccggataa cgggaaaagc attgaacacc ataggtcaga 23581 gtagtgacaa gtgttggcca cggaacaggt agttttccag tagtgcaaat aaatttaagg 23641 gtgagttttc cgtttgtagc atcaccttca ccctctccac ggacagaaaa tttgtgccca 23701 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct 23761 ttgctaccgc ttccacctcc acctggatcc aagcttccgc cgcctcctga accaccaccg 23821 ccactacctc ctcccccaga acctccgcct ccaccagcgt aatctggaac atcgtatggg 23881 tagctgctca cggtcaccag ggtgccctgg ccccagtagt cgaacaggcc ggtcacgcag 23941 tagtacaggg cggtgtcgtc gctgcgcacc ttgctcatct gcaggtacac ggtgttcttg 24001 ccgttgtcct tgctgatgat gaagcggtcc ttcagggcgc tgttgtagtc ggtgatgccg 24061 tcgccccaga tcacgccgat ccactccagg ccgcggccgg gggcctggcg cacccagttc 24121 acgccgtagt cggtcaggct gaagccgctc acggcgcagc tcagcttcag gctgccgccg 24181 ggctgcacca ggccgccgcc gctctccagc agcttcacct cgctgccgcc gccgctgctg 24241 ccgccgccgc cgctgccgcc gccgccgctg ccgccgccgc cgcgcttcag ctccaccttg 24301 gtgccctggc cgaacaccca gtggttgctg taccacaggg cgcagaagta ggtggcgaag 24361 tcctcgggct gcaggctgct gatggtcagg gtggccttgt cgccgatcag gctgccgctg 24421 aagcggctgg gcacgccggg ggcgcggttg ttggtgccgc cgatcaggcc cttgaacagc 24481 ttgccgggct tctcctgcac ccagctggcg tagttgctgg tggtcacggc gccggtgctg 24541 ctgcggcagg tgatggtcac gcggtcgccc acgctggcgc tcaggctgct ggggctctgg 24601 gtcatcacga tgtcggggcc cataaaccct aggtgttaat cagaaaaact cagattaatc 24661 gacaaattcg atcgcacaaa ctagaaacta acaccagatc tagatagaaa tcacaaatcg 24721 aagagtaatt attcgacaaa actcaaatta tttgaacaaa tcggatgata tctatgaaac 24781 cctaatcgag aattaagatg atatctaacg atcaaaccca gaaaatcgtc ttcgatctaa 24841 gattaacaga atctaaacca aagaacatat acgaaattgg gatcgaacga aaacaaaatc 24901 gaagattttg agagaataag gaacacagaa atttaccttg atcacggtag agagaattga 24961 gagaaagttt ttaagatttt gagaaattga aatctgaatt gtgaagaaga agagctcttt 25021 gggtattgtt ttatagaaga agaagaagaa aagacgagga cgactaggtc acgagaaagc 25081 taaggcggtg aagcaatagc taataataaa atgacacgtg tattgagcgt tgtttacacg 25141 caaagttgtt tttggctaat tgccttattt ttaggttgag gaaaagtatt tgtgctttga 25201 gttgataaac acgactcgtg tgtgccggct gcaaccactt tgacgccgtt tattactgac 25261 tcgtcgacaa ccacaatttc taacggtcgt cataagatcc agccgttgag atttaacgat 25321 cgttacgatt tatatttttt tagcattatc gttttatttt ttaaatatac ggtggagctg 25381 aaaattggca ataattgaac cgtgggtccc actgcattga agcgtatttc gtattttcta 25441 gaattcttcg tgctttattt cttttccttt ttgttttttt ttgccattta tctaatgcaa 25501 gtgggcttat aaaatcagtg aatttcttgg aaaagtaact tctttatcgt ataacatatt 25561 gtgaaattat ccatttcttt taatttttta gtgttattgg atatttttgt atgattattg 25621 atttgcatag gataatgact tttgtatcaa gttggtgaac aagtctcgtt aaaaaaggca 25681 agtggtttgg tgactcgatt tattcttgtt atttaattca tatatcaatg gatcttattt 25741 ggggcctggt ccatatttaa cactcgtgtt cagtccaatg accaataata ttttttcatt 25801 aataacaatg taacaagaat gatacacaaa acattctttg aataagttcg ctatgaagaa 25861 gggaacttat ccggtcctag atcatcagtt catacaaacc tccatagagt tcaacatctt 25921 aaacaagaat atcctgatct gaagaatgtg gaggctttag tcccttggat acttgggagg 25981 ctgtggaaga acagaaacga gctggtgctt aaagggaggg aatttggaac caatgaggta 26041 ttagtaagga caca //